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MALDI-tof

Kimki / 2022-08-08

  1. Fill out the MALDI excel form, put the name of the isolates (2 spot per isolate), then two spots for BTS (control).
  1. Apply samples on the plate, one sample has two consecutive spots.Also two spots BTS at the end.
  2. Add 1 uL 70% formic acid to each sample, but not BTS. Let the plate to be dry.
  3. Add 1 uL Matrix to each spot, including BTS. Let the plate to be dry.
  1. Open the MALDI-tof machine.
  2. Check if the excel IDs match the plate.
  3. Use BTS to adjust, check the machine is in good working condition.
  4. Start reading the samples and copy the result.